Fluigents unique broad range of solutions for use in microfluidic and nanofluidics applications ensure full control of flow rates with a greater control, automation, precision, ease of use and also minimize contamination. Fluigent has developed a pressure-based technology that offers an integrated pressure supply and control (positive and negative pressure) in a light (< 170 g) and compact format (L*l*H = 7*5*4). The MFCS series product range is the first generation of microfluidic systems. Continuous monitoring of water resources such as freshwater, seawater, and, in particular, wastewater and drinking water, for human and animal consumption, is essential2. 
The Fluigent LineUp series, including the new push-pull pump, enables precise and highly controlled aspiration and respiration of liquids. 
Our technology is an excellent fit for such applications as it is fully connected and provides excellent fluidic performance while being compact. In fact, it is possible to observe that the micropump will take more than a minute to reach zero pressure. The entire system can be controlled without a PC using local control and can also be monitored by Fluigent Software to extend its capabilities and benefit from automation. With the addition of a pressure leakage component, the pressure drop now takes about 5 seconds (see Fig. Most pressure controllers require a pressure source which is regulated. Pressure modules (positive pressure, negative pressure, or push-pull modules), microfluidic valve modules, and flow sensors are directly connected to the main platform. When combined with a FLOW UNIT, one can control flow rate directly or deliver dispensed volumes as needed. Due to its individually stackable channels adapted for different pressures, the PX series allowed for control of the different pressure variations required. The Flow EZmodules, are available in different pressure and vacuum ranges, to provide the optimum level of pressure control and resolution. Available in two main product line, the LineUpTM series to lead evolutive, compact and adaptable operations and the MFCSTM series for customized and field proven experience. The F-OEM makes use of the best combination of pneumatic, mechanical, and electrical elements associated with our new generation algorithm: Fluigent Direct Flow-rate Control (DFC) a self-learning algorithm that performs continuous adjustment over the algorithm parameters based on the actual response time. 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Control in real-time, protocol automation, data record and export. Along with the MCFS-EZ or MCFS-EX, a Manifold can be added to redirect the pressure to multiple fluid reservoirs. One can be operational and generate data rapidly. The module can be used to multiply the channel output number in the LineUp system or any other pressure system. You can choose the number of channels of the device (4 or 8), each channel range from -800 mbar to 7 bar, and you even have the possibility to integrate a pressure or vacuum source inside the instrument. The PX module is a CE and RoHS compliant single controller available in 3 pressure ranges. It provides a dual interface USB and RS232 for high versatility in the integration. How to set the Flow EZ (power and pressure supply), Fluidic and pneumatic connection to the microfluidic setup, Extend the pressure system by adding modules, How to send and apply an order to pressurize a reservoir, How to disconnect a module to modify the pressure system, Add a flow sensor to control directly in flow rate, Switch the module OFF and stop the experiment, 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, A human BBB (blood brain barrier)-on-chip to assess vascular permeability, Doing PhD with Fluigent 2021: Research on Honey by Daniel Kraus (Jena, Germany), Hans-Knll-Institut, New Antibiotics, Cultivation in Droplets, Microfluidic white paper An exploration of Microfluidics and fluid handling, LineUp Series, the new generation of microfluidic controllers, The Hebrew University: Single-cell encapsulation and culture in 3D hydrogels followed by InDrops / Drop-Seq microfluidic protocols for RNA profiling, University of Cambridge: Giant unilamellar vesicle production and testing, The Micro/Nano Bioelectronics and Biosensors (MBIOS) from Tianjin University, Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications, E. Coli culture in droplets using dSURF fluorosurfactant, Characterization of copper electrodeposition in liquid phase electron microscopy, Production of water-in-oil emulsions using a droplet generator chip, Droplet generation using syringe pumps and pressure-based flow controllers, Comparison between peristaltic, syringe and pressure pumps for microfluidic applications, University of Maryland: A soft robotic hand with integrated fluidic circuitry that can play Nintendo, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, 0,1% on the measured value (effective beyond 10% of the maximum pressure), Dry, oil-free gas, air, any non corrosive or non explosive gas, Aqueous solvent, oil, organic solent, biological sample, Control in real-time, protocol automation, data record and export. Each module consists of a separate pressure channel and can be controlled independently. Get full control of the setup in a single interface with plug and play capabilities available for all desktop OS, that allows control, monitoring, and automation feature. Bioeng. TheFlow EZis the most advanced system available forpressure-based flow control. The pressure automatically adjusts in the background to maintain the set flow rate. The technology used in this pressure pumpavoids cross contaminationbecause liquids only contact air. Combine as many modules as you want to fit your application needs. Configuration 3: 3 pressure modules 1 switch module. No switch modules. We have a large range of flow sensors ranging from 0-1.5 L/min to 0-5 mL/min. 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, Key indicators to ensure long-term performance of your OEM flow control components, Things you should know when integrating fluidics into your system, DFC, self-learning flow rate control algorithm, Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications, Droplet generation using syringe pumps and pressure-based flow controllers, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Pressure : 0 to 7000 mbar (101 psi) required pressure supply 7100 mbar (103 psi), Pressure: 0 to 2000 mbar (29 psi) required pressure supply 2100 mbar (30.4 psi) min, 2400 max, Pressure: 0 to 1000 mbar (14.5 psi) required pressure supply 1100 mbar (16 psi) min, 1300 max, Pressure: 0 to 345 mbar (5 psi) required pressure supply 1100 mbar (16 psi) (600 min, 1300 max), Pressure: 0 to 69 mbar (0.9 psi) required pressure supply 150 mbar (2.18 psi), Pressure: 0 to 25 mbar (0.36 psi) required pressure supply 150 mbar (2.18 psi), Vacuum : -25 mbar (-0.36 psi) / -69 mbar (-0.9 psi) / -345 mbar (-5 psi) / -800 mbar required vacuum -800 mbar (-11.6 psi), Push-Pull : -800 mbar (-11.6 psi) to 1000 mbar (14.5 psi) required pressure 1100 mbar (16 psi) min and 1300 max and vacuum -800 mbar (-11.6 psi) (min), 0.1% of full scale CV (on measured values), Liquid flow rate sensor input, customizable to work with third party sensors (through SDK), Control up to 4 x switches or valves per module compatible with Fluigent 2 position switches and rotary valves (M-switch, L-switch, 2-switch), Pressurized or bottled clean dry and non-corrosive or explosive gas (Ambient air, N2, Ar, CO2) (02 could be a thing, need to check), OD 4mm female push in fitting (on standard version, can be fittingless, then it is an M5 thread), >1W groundable for F-OEM platform (can be increased depending on peripherals, eg pump, fans etc) 6W maximum per FEZ module, 48W maximum per SWEZ module (rotary valves ~ 12W), External power, 2A or 7A available from standard, but custom can be accepted using MBPT terminal block connection, USB (standard board), RS232 (alternative board) + other protocols on demand, RJ45 female (or 2wire terminal block for SWEZ lite), 0-24VDC, digital controlled output 5 or 24 volts selectable, 2 x USB2.0 ports (available only on the USB connection protocol version, 0.1 Kg (F-OEM platform), 0.4 Kg (per pressure module with manifold), 0.3 kg (per switch module), Main electronic board. How to set the Flow EZ (power and pressure supply), Fluidic and pneumatic connection to the microfluidic setup, Extend the pressure system by adding modules, How to send and apply an order to pressurize a reservoir, How to disconnect a module to modify the pressure system, Add a flow sensor to control directly in flow rate, Switch the module OFF and stop the experiment, 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, LineUp Series, the new generation of microfluidic controllers, Comparison between peristaltic, syringe and pressure pumps for microfluidic applications, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, 0,1% on the measured value (effective beyond 10% of the maximum pressure), Dry, oil-free gas, air, any non corrosive or non explosive gas, Aqueous solvent, oil, organic solent, biological sample, Control in real-time, protocol automation, data record and export. The addition of aflow sensorenables one tocontrol or monitor flow rateas well asdispense volume. The Push-Pull allows for regulatation of pressure and vacuum down to -800 mbar and up to 1 bar from one single channel. The Flow EZ integrates the all-new DFC (Direct Flow Control) algorithm. Based on our industry leading experience, Fluigent has developed the patented QuadCore pneumatic system, the most advanced microfluidic pumping technology available. Instead of looking at the PC, users can keep their eyes on the microscope, adjusting the control dial with one hand. It is a standalone, modular platform that will perform complex fluidic operations. The FASTAB microfluidic patented technology used by MFCS series avoids cross contamination as there is no direct contact between the instrument and the reagents. Extension slots available, Pressure: 25 mbar (0.36 psi) / 69 mbar (0.9 psi) / 345 mbar (5 psi) / 1000 mbar (14.5 psi) /, USB cable, domino, electrical wires, 4 mm and 6 mm pneumatic tubing (4 m), Power supply 108 W if using a Switch module, Positive pressure source with necessary tubing and fittings, Negative pressure source with necessary tubing and fittings, Pressure regulator if pressure of different ranges are used. The sensor can be directly connected to a PC via USB, and display measurement in real time onthe Fluigent OxyGENsoftware interface. Our engineered systems tackle these limitations by bringing a cost-effective solution with a highly reduced footprint. TheLineUpP-SWITCHis a pneumatic valve controller. The technology consists of a uniquely engineered assembly of electronics, sensors integrated with a micropump, and pneumatics to provide high performance.
Mixed pressure ranges can be connected to the same board (positive and negative pressures, and Push-Pull). To ensure excellent response time while guaranteeing the best performance, Fluigent developed a technology that makes use of proportional valves. With the Fluigent Microfluidic Flow Control System (MFCS-4C) one can sort cells by size, shape and deformability as shown in this publication in the journal Lab-on-a-Chip and explained in the next video. Supports both pressure control and direct volume flow rate control depending on user needs. Our LineUp product range is the next generation of microfluidic systems. Point of Care (PoC) testing allows one to diagnose diseases at or near the patient site. The use of pressure allows for a quick response time as well. Users can output values for custom software applications using theSoftware Development Kit. It can be used without a PC or controlled with Fluigent Software Solutions to benefit from control in real-time, protocol automation, graphic displays and custom integration. The Push-Pull can maintain continuous, pulseless flow for days without refilling. It can easily be connected to other microfluidic devices such as FLOW UNIT sensors to allow for flow-rate control. This technology combines electronic and pneumatic elements engineered together with well established Fluigent regulation algorithms and knowledge. Combined with Flow EZ and Push-Pull, set a versatile system for pressure-base microfluidic experiments. Explore Fluigent industrial. Point of care tests such as blood analysis, glucose monitoring, infectious disease testing, cholesterol testing, or cardiac markers are marketed1. Reach your pressure targets rapidly and get your experiment started instantaneously. Customize your own controller to answer your control needs. 8, 114 (2021). The addition of aFLOW UNITenables one tocontrol or monitor flow rateas well asmeasure a dispensed volume. Excellent response time, pulseless and highly stable, Configurable with pressure and switch control modules, Standalone platform adapted to industrial uses. It is at the heart of the Fluigents next generation performance providing the fastest, most stable and compact system for microfluidics available. Custom software application: Public SDK libraries are available on GitHub for all integration into the users proprietary software, Adapted to cell culture or other experiments involving resistance variations: the algorithm adjusts its model to the setup resistance in real-time, Save precious sample or reagent: reduced time to reach desired flow rates uses less liquid during a calibration step, Save time with reduced settling time and no calibration. The product line can detect values over the range of-1000 mbar(-15 psi) to7000 mbar(100 psi). The Push-Pull integrates the all-new DFC (Direct Flow Control) algorithm. We offer best-in-class standard & custom OEM components, system development, and engineering services, with strong expertise in fluid management to accelerate your development process.
With a compact and modular design, the Push-Pull allows you to set and benefit quickly from the pressure-based flow control advantages for your experiments. The applied pressure will automatically adjust to maintain the flow rate. 6). The device is well designed and allows for easy control of my microfluidic chips. In this stand-alone configuration, the device allows for pressure or flow rate control and volume dispense making it ideal for benchtop use. A photonic technology institute tests a variety of lab-on-chip systems, the number of flow channels and the required pressures vary from one test to another. Fluigent was the first company to solve this problem by introducing an innovative technology: pressure pumps. The principle of pressure actuation in microfluidic systems is shown in the figure. The LineUp series module can be combined together to add pressure/vacuum channels, valve control and turn one channel into 8 independent outlets using the P-SWITCH. As the equipment is not easily portable, samples can be compromised during travel. If you are seeking to replace high-precision syringe pumps or other conventional instruments, we offer modern microfluidic systems and components that enhance productivity.. The algorithm includes a continuous optimization of the parameters that allow it to adapt to the interactions between microfluidic channels in complex situations. Fig 2: (left) Pneumatic schematic of a standard fluidic system using pressure to move fluids (right) Pneumatic schematic of Fluigent new technology. TheLineUpPush-Pullis a standalone controller with the ability to deliverfinely regulated pressureor avacuumthrough a single outlet over the range of-800 to +1000 mbar. Each channel can be controlled independently and deliver a specific amount of pressure or vacuum to handle fluids. 1186, (2021). 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, Success story of SEED Biosciences : Single-cell injection and impedance analysis, University of Cambridge: Giant unilamellar vesicle production and testing, The Micro/Nano Bioelectronics and Biosensors (MBIOS) from Tianjin University, Microbiome culture in droplet using dsurf surfactant, Droplet and particle manipulation using electrophoretic flow control, Cartilage on chip using Fluigent MFCS pressure controller, Fluid recirculation for cell perfusion with reduced shear stress, Droplet generation using syringe pumps and pressure-based flow controllers, Extended capabilities of pressure driven flow for microfluidics applications, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Highly monodispersed droplet size No oscillation, No transitory regime Reagent saving Stop-flows, Wide range of volume can be controlled No compromise between volume and stability, Any mix or pressure ranges are available Highly customizable, Female luer lock (-800 to 2000 mbar) 4mm OD tube (7 bar), Dry, oil-free gas, air, any non corrosive or non explosive gas, Aqueous solvent, oil, organic solent, biological sample, Control in real-time, protocol automation, data record and export. The F-OEM makes use of Fluigents patented Fastab 2 technology. 5 that compares Fluigent solution with a standalone gas micropump. Improved reliability and reproducibility of results are possible due to the pulseless flow. 67 avenue de Fontainebleau94 270 Le Kremlin-Bictre, Things you should know when integrating fluidics into your system, Peristaltic pump vs pressure-based microfluidic flow control systems for Organ on-chip applications, Droplet generation using syringe pumps and pressure-based flow controllers, Extended capabilities of pressure driven flow for microfluidics applications, Comparison between peristaltic, syringe and pressure pumps for microfluidic applications, Pressure predictions for lab-on-a-chip operations using a microfluidic network solver and Fluigent PX, All-in-one pressure supply & pressure/flow control, Advantages of pressure-based microfluidics, Droplet and particle generation in microfluidics, Control in real-time, protocol automation, data record and export. A flow sensor can directly be connected to the engineered device. Control and regulate pressure and flow rate without a PC using the LineUp hardware interface. The Adapt is used to connect Flow EZ modules with different pressure ranges without the need of additional pressure sources. All-in-one pressure controller and supply powered by battery or line voltage. Its patented, field-proven Fastab technology allows both fast settling times and outstanding stability. The pressure automatically adjusts in the background to maintain the set flow rate. Connect the outlets of the P-SWITCH directly to quake or on-chip pneumatic valves to actuate them. It can support a wide range of connections (Wi-Fi, BLE, IoT, USB, industrial bus), and embedded protocols. 1. The MFCSTM series and Flow EZTM with Fluigent valves can be used for automated cell perfusion as in the next video. The F-OEM range of modules allows for regulation of vacuum/pressure down to -800 mbar and up to 7 bar, with the possibility to use a push-pull module (-800 / +1 000 mbar). is well-suited technology for applications that require mobile or compact fluidic control devices, including point of care (PoC) diagnostic and testing, or for water and soil on-site analysis. Using this engineered solution, pressure drop takes less than a second, and the maximum pressure reachable has not deteriorated (see graph). Liquid flow rate ranges from a few nL/min to 5 mL/min. There are many advantages to direct control: Configuration 1: 2 pressure modules. We provide instrument development industrial engineers with the right liquid handling solution for your system. ThePXmodules, are available in different pressure and vacuum ranges, to provide the optimum level of pressure control and resolution. Based on our industry leading experience, Fluigent has developed the patented QuadCore pneumatic system, the most advanced microfluidic pumping technology available. Combine up to 8 pressure modules on a single F-OEM platform. What I like most is that you are independent of a computer and can directly control both positive and negative pressure..